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CABRI:DSMZ_MUTZ |
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Accession_number |
ACC 39
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Cell_line_name |
380
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Brief_description |
confirmed as human with IEF of MDH, NP established from the peripheral blood of a
15-year-old boy with acute lymphoblastic leukemia (ALL FAB L2) in relapse in 1983;
cells were described to be EBNA-negative and to carry (8;14) and (14;18)
translocations
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Description |
human B cell precursor leukemia established from the peripheral blood of a 15-year-
old boy with acute lymphoblastic leukemia (ALL FAB L2) in relapse in 1983; cells were
described to be EBNA-negative and to carry (8;14) and (14;18) translocations
confirmed as human with IEF of MDH, NP
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Depositor |
Dr. L. Pegoraro, University of Torino, Turin, Italy
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Morphology |
round, small, single cells in suspension
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Culture_conditions |
80-90% RPMI 1640 + 10-20% FBS cells are very difficult to culture and grow very
slowly! cells appear to grow better in 24-well-plates than in flasks; seed out at ca. 1 x
10 6 cells/ml; optimal density at about 1-3 x 106 cells/ml; split carefully at 37C with
5% CO2 maximal density at about 4-5 x 106 cells/ml
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Viruses |
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV -,
HTLV-I/II -, SMRV -
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Properties |
CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, cyCD79a +, CD80 -,
CD138 (+), HLA-DR +, sm/cyIgM -, sm/cyIgG -, sm/cykappa -, sm/cylambda -
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Release_conditions |
-
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Hazard |
-
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Storage |
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule
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Doubling_time |
ca. 80 hours
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Mycoplasma |
negative in DAPI, microbiological culture, PCR assays
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Fingerprint |
multiplex PCR of minisatellite markers revealed a unique DNA profile
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Cytogenetics |
human near-diploid karyotype with 1.8% polyploidy - 46(43-47)<2n>XY, -14,
t(8;14;18)(q24;q32;q21), +der(14)t(8;14;18)(q24;q32;q21) - corresponds to published
karyotype
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Bibliographic_reference |
Pegoraro et al., Proc Natl Acad Sci USA, 81: 23 [PMID: 6334305
] |
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